Anti-inflammatory Effects of Extracts of Sweet Basil (Ocimum basilicum L.) on a Co-culture of 3T3-L1 Adipocytes and RAW264.7 Macrophages.
Identifieur interne : 000212 ( Main/Exploration ); précédent : 000211; suivant : 000213Anti-inflammatory Effects of Extracts of Sweet Basil (Ocimum basilicum L.) on a Co-culture of 3T3-L1 Adipocytes and RAW264.7 Macrophages.
Auteurs : Haruka Takeuchi ; Chie Takahashi-Muto ; Mana Nagase ; Masahiro Kassai ; Rieko Tanaka-Yachi ; Chikako KiyoseSource :
- Journal of oleo science [ 1347-3352 ] ; 2020.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), Adipocytes (métabolisme), Animaux (MeSH), Anti-inflammatoires (MeSH), Antigènes CD137 (génétique), Antigènes CD137 (métabolisme), Cellules 3T3 (MeSH), Cellules RAW 264.7 (MeSH), Cytokines (génétique), Cytokines (métabolisme), Expression des gènes (effets des médicaments et des substances chimiques), Extraits de plantes (pharmacologie), Facteur de transcription NF-kappa B (génétique), Facteur de transcription NF-kappa B (métabolisme), Médiateurs de l'inflammation (métabolisme), Ocimum (composition chimique), Souris (MeSH), Techniques de coculture (MeSH).
- MESH :
- composition chimique : Ocimum.
- effets des médicaments et des substances chimiques : Expression des gènes.
- génétique : Antigènes CD137, Cytokines, Facteur de transcription NF-kappa B.
- métabolisme : ARN messager, Adipocytes, Antigènes CD137, Cytokines, Facteur de transcription NF-kappa B, Médiateurs de l'inflammation.
- pharmacologie : Extraits de plantes.
- Animaux, Anti-inflammatoires, Cellules 3T3, Cellules RAW 264.7, Souris, Techniques de coculture.
English descriptors
- KwdEn :
- 3T3 Cells (MeSH), Adipocytes (metabolism), Animals (MeSH), Anti-Inflammatory Agents (MeSH), Coculture Techniques (MeSH), Cytokines (genetics), Cytokines (metabolism), Gene Expression (drug effects), Inflammation Mediators (metabolism), Mice (MeSH), NF-kappa B (genetics), NF-kappa B (metabolism), Ocimum (chemistry), Plant Extracts (pharmacology), RAW 264.7 Cells (MeSH), RNA, Messenger (metabolism), Tumor Necrosis Factor Receptor Superfamily, Member 9 (genetics), Tumor Necrosis Factor Receptor Superfamily, Member 9 (metabolism).
- MESH :
- chemical , genetics : Cytokines, NF-kappa B, Tumor Necrosis Factor Receptor Superfamily, Member 9.
- chemical , metabolism : Cytokines, Inflammation Mediators, NF-kappa B, RNA, Messenger, Tumor Necrosis Factor Receptor Superfamily, Member 9.
- chemical , pharmacology : Plant Extracts.
- chemical : Anti-Inflammatory Agents.
- chemistry : Ocimum.
- drug effects : Gene Expression.
- metabolism : Adipocytes.
- 3T3 Cells, Animals, Coculture Techniques, Mice, RAW 264.7 Cells.
Abstract
Obesity, a lifestyle disease resulting from excessive caloric intake and insufficient physical activity, results in a state of chronic inflammation. A food ingredient that suppresses chronic inflammation could help prevent associated diseases. Sweet basil (Ocimum basilicum L.) is a herb from the Lamiaceae family with some reported anti-inflammatory effects. Via this in vitro study, we aimed to investigate whether sweet basil exerts anti-inflammatory effects in obese patients. Fresh sweet basil leaves were freeze-dried and powered. After that, this was extracted with 80% methanol. After 3T3-L1 adipocytes were cultured with sweet basil extracts at final concentrations of either 5 or 25 μg/mL for 24h, RAW264.7 macrophages were seeded onto this adipocytes and co-cultured for 12h. We determined the effects of sweet basil extracts on inflammatory cytokine expression by real-time PCR or western blotting. Sweet basil extracts reduced the expression of inflammatory cytokine mRNA induced by co-culture, including that of IL-6 (Il6), IL-1β (Il1b), TNF-α (Tnf), and CCL2 (Ccl2). In addition, sweet basil extracts suppressed the mRNA expression of NF-κB (Nfκb1), a transcription factor of inflammatory cytokines. In an investigation of costimulatory CD137 (Tnfrsf9)/CD137L inflammatory signaling, a member of the TNF super-family, sweet basil extracts inhibited Tnfrsf9 expression induced by the co-culture. Therefore, the results of this study indicated that sweet basil extracts have an anti-inflammatory effect against adipocyte-induced inflammation, possibly through suppression of Tnfrsf9 expression.
DOI: 10.5650/jos.ess19321
PubMed: 32281564
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<term>Adipocytes (metabolism)</term>
<term>Animals (MeSH)</term>
<term>Anti-Inflammatory Agents (MeSH)</term>
<term>Coculture Techniques (MeSH)</term>
<term>Cytokines (genetics)</term>
<term>Cytokines (metabolism)</term>
<term>Gene Expression (drug effects)</term>
<term>Inflammation Mediators (metabolism)</term>
<term>Mice (MeSH)</term>
<term>NF-kappa B (genetics)</term>
<term>NF-kappa B (metabolism)</term>
<term>Ocimum (chemistry)</term>
<term>Plant Extracts (pharmacology)</term>
<term>RAW 264.7 Cells (MeSH)</term>
<term>RNA, Messenger (metabolism)</term>
<term>Tumor Necrosis Factor Receptor Superfamily, Member 9 (genetics)</term>
<term>Tumor Necrosis Factor Receptor Superfamily, Member 9 (metabolism)</term>
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<term>Adipocytes (métabolisme)</term>
<term>Animaux (MeSH)</term>
<term>Anti-inflammatoires (MeSH)</term>
<term>Antigènes CD137 (génétique)</term>
<term>Antigènes CD137 (métabolisme)</term>
<term>Cellules 3T3 (MeSH)</term>
<term>Cellules RAW 264.7 (MeSH)</term>
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<term>Tumor Necrosis Factor Receptor Superfamily, Member 9</term>
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<term>Facteur de transcription NF-kappa B</term>
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<term>Facteur de transcription NF-kappa B</term>
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<front><div type="abstract" xml:lang="en">Obesity, a lifestyle disease resulting from excessive caloric intake and insufficient physical activity, results in a state of chronic inflammation. A food ingredient that suppresses chronic inflammation could help prevent associated diseases. Sweet basil (Ocimum basilicum L.) is a herb from the Lamiaceae family with some reported anti-inflammatory effects. Via this in vitro study, we aimed to investigate whether sweet basil exerts anti-inflammatory effects in obese patients. Fresh sweet basil leaves were freeze-dried and powered. After that, this was extracted with 80% methanol. After 3T3-L1 adipocytes were cultured with sweet basil extracts at final concentrations of either 5 or 25 μg/mL for 24h, RAW264.7 macrophages were seeded onto this adipocytes and co-cultured for 12h. We determined the effects of sweet basil extracts on inflammatory cytokine expression by real-time PCR or western blotting. Sweet basil extracts reduced the expression of inflammatory cytokine mRNA induced by co-culture, including that of IL-6 (Il6), IL-1β (Il1b), TNF-α (Tnf), and CCL2 (Ccl2). In addition, sweet basil extracts suppressed the mRNA expression of NF-κB (Nfκb1), a transcription factor of inflammatory cytokines. In an investigation of costimulatory CD137 (Tnfrsf9)/CD137L inflammatory signaling, a member of the TNF super-family, sweet basil extracts inhibited Tnfrsf9 expression induced by the co-culture. Therefore, the results of this study indicated that sweet basil extracts have an anti-inflammatory effect against adipocyte-induced inflammation, possibly through suppression of Tnfrsf9 expression.</div>
</front>
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<DateCompleted><Year>2020</Year>
<Month>09</Month>
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<Title>Journal of oleo science</Title>
<ISOAbbreviation>J Oleo Sci</ISOAbbreviation>
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<ArticleTitle>Anti-inflammatory Effects of Extracts of Sweet Basil (Ocimum basilicum L.) on a Co-culture of 3T3-L1 Adipocytes and RAW264.7 Macrophages.</ArticleTitle>
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<Abstract><AbstractText>Obesity, a lifestyle disease resulting from excessive caloric intake and insufficient physical activity, results in a state of chronic inflammation. A food ingredient that suppresses chronic inflammation could help prevent associated diseases. Sweet basil (Ocimum basilicum L.) is a herb from the Lamiaceae family with some reported anti-inflammatory effects. Via this in vitro study, we aimed to investigate whether sweet basil exerts anti-inflammatory effects in obese patients. Fresh sweet basil leaves were freeze-dried and powered. After that, this was extracted with 80% methanol. After 3T3-L1 adipocytes were cultured with sweet basil extracts at final concentrations of either 5 or 25 μg/mL for 24h, RAW264.7 macrophages were seeded onto this adipocytes and co-cultured for 12h. We determined the effects of sweet basil extracts on inflammatory cytokine expression by real-time PCR or western blotting. Sweet basil extracts reduced the expression of inflammatory cytokine mRNA induced by co-culture, including that of IL-6 (Il6), IL-1β (Il1b), TNF-α (Tnf), and CCL2 (Ccl2). In addition, sweet basil extracts suppressed the mRNA expression of NF-κB (Nfκb1), a transcription factor of inflammatory cytokines. In an investigation of costimulatory CD137 (Tnfrsf9)/CD137L inflammatory signaling, a member of the TNF super-family, sweet basil extracts inhibited Tnfrsf9 expression induced by the co-culture. Therefore, the results of this study indicated that sweet basil extracts have an anti-inflammatory effect against adipocyte-induced inflammation, possibly through suppression of Tnfrsf9 expression.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Takeuchi</LastName>
<ForeName>Haruka</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Graduate school of Kanagawa Institute of Technology.</Affiliation>
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<Author ValidYN="Y"><LastName>Takahashi-Muto</LastName>
<ForeName>Chie</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Department of Clinical Nutrition, Kitasato Junior College of Health and Hygicnic Sciences.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nagase</LastName>
<ForeName>Mana</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Department of Nutrition and Life Science, Kanagawa Institute of Technology.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kassai</LastName>
<ForeName>Masahiro</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>S&B food Inc.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Tanaka-Yachi</LastName>
<ForeName>Rieko</ForeName>
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<AffiliationInfo><Affiliation>Department of Applied Biochemistry, Kanagawa Institute of Technology.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kiyose</LastName>
<ForeName>Chikako</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Graduate school of Kanagawa Institute of Technology.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Nutrition and Life Science, Kanagawa Institute of Technology.</Affiliation>
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<MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName>
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<Keyword MajorTopicYN="N">inflammation</Keyword>
<Keyword MajorTopicYN="N">macrophages</Keyword>
<Keyword MajorTopicYN="N">obesity</Keyword>
<Keyword MajorTopicYN="N">sweet basil</Keyword>
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<ArticleIdList><ArticleId IdType="pubmed">32281564</ArticleId>
<ArticleId IdType="doi">10.5650/jos.ess19321</ArticleId>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Kassai, Masahiro" sort="Kassai, Masahiro" uniqKey="Kassai M" first="Masahiro" last="Kassai">Masahiro Kassai</name>
<name sortKey="Kiyose, Chikako" sort="Kiyose, Chikako" uniqKey="Kiyose C" first="Chikako" last="Kiyose">Chikako Kiyose</name>
<name sortKey="Nagase, Mana" sort="Nagase, Mana" uniqKey="Nagase M" first="Mana" last="Nagase">Mana Nagase</name>
<name sortKey="Takahashi Muto, Chie" sort="Takahashi Muto, Chie" uniqKey="Takahashi Muto C" first="Chie" last="Takahashi-Muto">Chie Takahashi-Muto</name>
<name sortKey="Takeuchi, Haruka" sort="Takeuchi, Haruka" uniqKey="Takeuchi H" first="Haruka" last="Takeuchi">Haruka Takeuchi</name>
<name sortKey="Tanaka Yachi, Rieko" sort="Tanaka Yachi, Rieko" uniqKey="Tanaka Yachi R" first="Rieko" last="Tanaka-Yachi">Rieko Tanaka-Yachi</name>
</noCountry>
</tree>
</affiliations>
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